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. 2011 Jan 5;1(3):356–361.

Figure 2.

Figure 2

Effect of PPP on IGF-1Rβ phosphorylation and downstream signaling pathways. A, Tyrosine-phosphorylated proteins were immunoprecipitated using the PY20 antibody from H295R cells and IGF-1Rβ revealed by immunoblotting (top panel), in basal conditions or after pre-incubation with 1 µM PPP for 6 hours. Total IGF-1Rβ expression is also shown (bottom panel). B, Expression of phospho-Akt, total Akt, phospho-ERK1/2, total ERK1/2 in H295R and SW-13 cells was analysed by immunoblot of serum-starved cells incubated with or without 10 µM PPP or NVP-AEW541 during 2h and stimulated with serum for 10, 30 or 60 min.