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. 2011 Jul 1;2(4):395–404. doi: 10.4161/mabs.2.4.12303

Figure 8.

Figure 8

Chromatogram of direct capture of mAb02 (0.62 mg/mL) from diluted cell culture supernatant on Eshmuno™ S (A). A 1 mL Scout column was loaded to 5% breakthrough. Experiments were performed with binding buffer pH 6 containing 20 mM phosphate and 20 mM NaCl (conductivity 4.3 mS/cm) at a residence time of 5 min. Bound protein was eluted by a linear gradient from binding buffer to elution buffer containing 20 mM phosphate and 0.8 M NaCl in 20 column volumes. Sample, flowthrough fractions (FT) and the pooled elution fractions (El) were analysed by SDS page under non-reducing conditions (B).