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. 2011 Jul 1;2(4):395–404. doi: 10.4161/mabs.2.4.12303

Table 3.

Dynamic binding capacities1 of Toyopearl GigaCap S, Fractogel SO3− and Eshmuno S for the capture of mAbs from diluted cell culture supernatant (CCS)

mAb03 CCS (mg/mL resin) mAb03 purified (mg/mL resin) mAb02 CCS (mg/mL resin) mAb02 purified (mg/mL resin)
Eshmuno S 89 90 96 109
Fractogel SO3 69 77 70 76
Toyopearl GigaCap S 64 83 64 88
1

Capacities were determined by breakthrough analysis with buffer containing 20 mM phosphate and 20 mM NaCl (conductivity 4.3 mS/cm). Experiments were performed at a residence time of 5 min (mAb02; 0.62 mg/mL; 5% breakthrough) or 2 min (mAb03; 1.5 mg/mL; 10% breakthrough) at pH 6 or pH 5.5, respectively.