Figure 4.

Stability of the glycosylation profile of EB66-produced monoclonal antibodies. An individual EB66 clone producing the anti-X antibody was selected for its production yields and expanded under fixed culture conditions for 105 generations. Monoclonal antibody batches were purified with EB66 cultures arrested at generation 1, 43 and 105 and were analyzed for their N-linked oligosaccharides composition (A and C). In parallel, an analysis of glycosylation was performed on purified antibody batches prepared from the same EB66 producer clone grown under three fedbatch conditions differing in the feeding strategies and leading to different production yields (B and D).