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. 2011 Jul 1;2(4):416–427. doi: 10.4161/mabs.2.4.12438

Figure 7.

Figure 7

Reactivity of parental mAbF1546 and recombinant FabF1546 to a crude extract of P. falciparum antigenic extract in western blots. A crude P. falciparum antigenic extract (lanes 1 and 2) and the periplasmic fluid of induced HB2151 (pER1) expressing MalE-PfHRP2 (lanes 3 and 4) were subjected to SDS-PAGE on a 7.5–15% polyacrylamide gel run under non reducing conditions. They were probed with the parental mAbF1546 (lanes 2 and 4, ascitic fluid at a 1/1,000 dilution) and the recombinant FabF1546-H6 (lanes 1 and 3, periplasmic fraction at a 1/10 dilution).