Figure 4.

Human osteoclasts generated from cryopre-served bone marrow in a simplified protocol exhibit resorptive capability. Human osteoclasts were generated on slices of devitalized bovine cortical bone. Pre -osteoclasts were cultured in the presence of 10 ng/ml recombinant human M-CSF and 100 ng/ml recombinant murine RANKL (right column) or without RANKL (left column) as a control. After 18 days, bone slices were subjected to SEM, which revealed the presence of resorption pits in RANKL-containing wells. Shown are representative micrographs at both 10IX and 406X magnification.