Figure 2.

GSH contents in MRP1-expressing cells. GSH contents in MRP1-expressing cells. A. GSH contents in MRP1-expressing cells are significantly lower than in their corresponding control cells. Total intracellular GSH was determined in triplicate [n = 55 (CFTR), 4 (K1333L), 65 (MRP1), 50 (H69) and 50 (H69AR)] according to the method described in Materials and Methods. ** indicates that the P value is less than 0.0001. B. Effects of 10 μM verapamil on the intracellular GSH contents. C and D. Effects of 10 μM BSO on the intracellular GSH contents. E and F. Effects of 2 μM daunomycin on the intracellular GSH contents. 10⁶ cells were plated out in each 35 mm dish and incubated overnight. The agents mentioned above were added to the media and incubated at 37 ° C for varying durations.