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. 2010 Oct 20;1(1):71–84.

Figure 3.

Figure 3

Modulation of E-cadherin and Slug expression in E-cad+ cells during invasion. (A) and (B) Invaded E-cad+ cells immunostained for E-cadherin. After 4 h of culture (t=4 h), top-chamber (non-invaded) cells were removed, and the invasion chambers were incubated for an additional 5, 10, or 15 h. At each time point, DU145 (A) and PC3 (B) cells were immunostained for E-cadherin. Magnification, 40x. (C) The relative Slug expression levels in E-cad+ cells from DU145 and PC3 cells residing in the top chambers of invasion assays were evaluated by qPCR at various time points (0, 2, 4, 8,16 and 24 h). Expression levels were normalized to actin. The data are reported as the mean ± SD.