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. 2011 Aug 1;10(15):2540–2548. doi: 10.4161/cc.10.15.16309

Figure 4.

Figure 4

p27 protein turnover contributes to the differential growth response of eMYC and iMYC cells to TGFβ. (A) Western analysis of protein lysates from empty vector control (pB), eMYC (MYC) and iMYC (iMYC) cells treated with TGFβ1 ligand. Levels of p27 and GAPDH are shown. (B) Western analysis of protein lysates from empty vector control (pB) and eMYC (MYC) cells transfected with either non-targeting siRNA (−) or against p27 (+). Levels of p27 and GAPDH are shown. (C) eMYC cells transfected with siRNA against p27 were plated 24 h after transfection, treated with 0.1 ng/ml TGFβ1 ligand and counted at 3 d after treatment. Levels of growth in TGFβ1-untreated and treated cells are shown relative to levels of growth in eMYC cells transfected with control siRNA and treated with ligand. (D) Western analysis of protein lysates from empty vector control (pB), eMYC (eMYC) and iMYC (iMYC) cells treated with TGFβ1 ligand, MG132, or both 2 h prior to protein harvest. Levels of p27 and GAPDH are shown.