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. 2011 Sep 9;11:31. doi: 10.1186/1475-2867-11-31

Figure 4.

Figure 4

Effects of tamoxifen, 4-hydroxytamoxifen, and deficiency of D-(+)-glucose, L-leucine, L-methionine or L-cysteine on the phosphorylation of 4E-BP1 in MDA-MB-231 cells. (a and e) Western immunoblot analysis of the effects of D-(+)-glucose deficiency, DMSO, tamoxifen and 4-hydroxytamoxifen on the expression of total 4E-BP1 (eukaryotic translation initiation factor 4E binding protein 1) and (b and e) phosphorylated 4E-BP1. (c and e) Western immunoblot analysis of the effects of the deficiency of L-leucine, L-methionine, or L-cysteine on the expression of total 4E-BP1 and (d and e) phosphorylated 4E-BP1. All assays were performed in triplicates and repeated three times. (f) Correlation between the degree of expression of p27 protein and the degree of down-regulation of the phosphorylated 4E-BP1.