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Effects of tamoxifen, 4-hydroxytamoxifen and deficiency of D-(+)-glucose on the expression of HIF-1a, SREBP-1 and phosphorylation of eEF2k in MDA-MB-231 cells. (a and e) Western immunoblot analysis of the effects of D-(+)-glucose deficiency, DMSO, tamoxifen and 4-hydroxytamoxifen on the expression of HIF1α. (b and e) SREBP-1, (c and e) total eEF2k and (d and e) phosphorylated eEF2k. All assays were performed in triplicates and repeated three times.
