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. 2011 Sep 9;11:31. doi: 10.1186/1475-2867-11-31

Figure 7.

Figure 7

Effects of tamoxifen, 4-hydroxytamoxifen and deficiency of D-(+)-glucose, L-leucine, L-methionine or L-cysteine on the expression of mitochondrial ATP5A and SIRT3 in MDA-MB-231 cells. (a and e) Western immunoblot analysis of the effects of D-(+)-glucose deficiency, DMSO, tamoxifen, 4-hydroxytamoxifen and (b and e) deficiency of L-leucine, L-methionine or L-cysteine on the expression of mitochondrial ATP5a in the Complex V of respiratory oxidation-phosphorylation system. (c and e) Western immunoblot analysis of the effects of D-(+)-glucose deficiency, DMSO, tamoxifen, 4-hydroxytamoxifen and (d and e) deficiency of L-leucine, L-methionine or L-cysteine on the expression of mitochondrial SIRT3. The SIRT3 is one of the seven mammalian anti-aging and anti-metabolic sirtuins. All assays were performed in triplicates and repeated three times.