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. 2011 Sep 26;6(9):e25020. doi: 10.1371/journal.pone.0025020

Figure 1. Pb2+ enhances Tau244–372 fibrillization.

Figure 1

(A) 10 µM Tau244–372 was incubated with 0–40 µM Pb2+ (black: 0 µM, red: 5 µM, blue: 10 µM, yellow: 20 µM, green: 30 µM, and magenta: 40 µM) in 10 mM HEPES buffer (pH 7.4) containing 100 mM NaCl, 1 mM DTT, 2.5 µM heparin, and 20 µM ThT, and ThT binding assays were carried out at 37°C. Data are expressed as mean ± S.D. (n = 3–4). (B) 10 µM Tau244–372 was incubated with 0–10 µM Pb2+ (black: 0 µM, and blue: 10 µM) in 10 mM HEPES buffer (pH 7.4) containing 1 mM DTT, 2.5 µM heparin and 20 µM ANS, and ANS binding assays were carried out at 37°C.