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. 2011 Sep 26;6(9):e25216. doi: 10.1371/journal.pone.0025216

Figure 9. Overexpressor mutants Oserf3 and Osap2-39 display decreased expression of ethylene biosynthesis genes and ethylene production.

Figure 9

Detection of the expression of ethylene biosynthesis genes detected by Q-PCR and ethylene emission in overexpressor mutants (A) and in overexpressing OsERF3 transgenic lines (B). The expression level of each gene in the Nipponbare or Zhonghua 11 (Zh11) was standardized to 1, referring to the internal control of OsActin1. Oserf3 and Osap2-39 are activation-tagged T-DNA insertion mutants in Zh11. The ethylene emission, average of three independent biological assays plus SD, is in terms of the increase over controls (sealed vials without any seedlings). nl/gFW.h indicates the amount of ethylene per gram fresh weight seedling in an hour. Student's t-tests were conducted using the data in transgenic seedlings compared to these in Nipponbare. * and ** indicate significant difference at P<0.05 and P<0.01 probability, respectively.