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. 2011 Aug 31;11:122. doi: 10.1186/1471-2229-11-122

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Copyright ©2011 Zienkiewicz et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fluorescence microscopy localization of caleosin in the olive anther. Sections from olive anthers at the PMC (A), Te (B), Mi (C), YP (D), MBP (E) and MP (F) stages were incubated with a FL anti-clo3 Ab, followed by an anti-rabbit IgG-Alexa Fluor 488-conjugated secondary Ab. Differential interference contrast (DIC) images of serial sections were also obtained to better visualize the different tissues of the anther. En: endothecium; Ep: epidermis; L: anther locule; MBP: mid bicellular pollen grain; Mi: microspore; MP: mature pollen grain; PMC: pollen mother cell; T: tapetum; Te: tetrad; YP: young pollen grain.