Figure 6. Alteration of preterminal axons of motor endplates in 4.1B KO mice.

In toto immunostaining of neuromuscular junctions on preparations of teased fibers from WT (A, B) and KO (C, D) mice with rhodamine-conjugated α-bungarotoxin (red) and antibodies directed against neurofilament subunit NF-M (green). NF-M labeling revealed irregular caliber with dilated segments in myelinated preterminal axons of the KO mouse (C, D, arrowheads). Scale bars: A, C, 75 µm; B, D, 15 µm.