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. 2011 Sep 26;6(9):e25255. doi: 10.1371/journal.pone.0025255

Figure 3. Clonal and allele-specific expression of XIST SNPs and MECP2 in female human iPSC lines.

Figure 3

(A) Representative sequencing traces of SNP analysis to distinguish allele-specific transcription of XIST in iPS-R294X-MT-1, iPS-R294X-WT, iPS-R306C-MT, iPS-R306C-WT, and iPS-T158M-WT. SNP rs1894271 (C or T) was examined in iPS-R294X-MT-1, iPS-R294X-WT. SNP rs16992442 (C or T) was examined in iPS-R306C-MT, iPS-R306C-WT, and iPS-T158M-WT. (B) Representative sequencing traces of regions of the MECP2 gene that contain RTT mutations to distinguish allele-specific transcription of MECP2 in iPS-R294X-MT-1, iPS-R294X-WT, iPS-R306C-MT, iPS-R306C-WT, and iPS-T158M-WT, iPS-V247X-MT, and iPS-V247X-WT. MT denotes expression of the mutant MECP2 allele. WT denotes expression of the wild type MECP2 allele. (C) Representative sequencing traces of the region of the MECP2 gene that contains RTT mutation V247X (705delG) to distinguish allele-specific transcription of MECP2 at the stages of embryoid body, neuroepithelia and neurosphere during directed neural differentiation from iPS-V247X-MT.