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Treatment with PKA inhibitor abolishes the enhancement of testosterone production by menaquinone-4 in I-10 cells. I-10 cells were treated with menaquinone-4 (MK-4) and protein kinase A inhibitor H89 simultaneously for 24 h, and then testosterone levels in the culture medium were determined by ELISA. Data are represented as means ± SE (n = 3). Values with different letters are significantly different at p < 0.05.
