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. 2011 Sep 26;6(9):e25484. doi: 10.1371/journal.pone.0025484

Figure 2. Transcription of rovA upon temperature shift from 26 to 37°C.

Figure 2

Two oligonucleotide primers were designed to be complementary to the RNA transcript of rovA. The primer extension products were analyzed with 8 M urea−6% acrylamide sequencing gel. Lanes C, T, A, and G represent the Sanger sequencing reactions. Only the WT strain was tested to grow under conditions I, II and III, respectively. Bacterial cells were harvested at the middle-exponential (a) or stationary (b) phase. Temperature upshift was designed prior to cell harvest, generating two kinds of cultures: ‘26°C continuously’ (&) and ‘shift from 26 to 37°C’ ( #). Detected were the two promoters P1 and P2 located at 78 (nucleotide T ) and 343 (G) bp upstream of rovA, respectively. Images shown are representative of the results from at least three biological replicates.