Table 1.
SSR primer sequences.
| SSR locus | GenBank accession | SSR Primer Sequence (F/R) | Product size (bp)a | # Positive plate pools |
|---|---|---|---|---|
| C127GAC1 | GF102017 | GCGTTTGGAGAGTGGAAAAG | 307 | 10 |
| TGAGTTTTCAGTGCCCTCCT | ||||
| C32AC1B | GF110611 | AAACGATCGCAATTCGAAAC | 242 | 3 |
| GTGGCGAACAACATTCACAG | ||||
| C32TC1B | GF110612 | TCATCGTTCATTCGTTCCAA | 179 | 2 |
| TGTCATTTTCTGCCTGCATC | ||||
| C32TG1 | GF102044 | CGTGTTTTCCATTGTTGGTG | 400 | 29 |
| TTGGCTGTGTCAACTGCTTC | ||||
| C68ACAT1 | GF102032 | GGCCTTGCTGAGAAAACTTG | 425 | 1 |
| CCCAAAATCCAAGCTTCAAA | ||||
| C68CA1 | GF102033 | ATAAAGCGACCAAGGCTCAA | 294 | 7 |
| GCGAAACCATTCAAAAGCAT | ||||
| C68GA1B | GF110613 | CACCCCACAGTTAACCCAAC | 245 | 8 |
| CTCGAGAGATTTGGCCTTTG | ||||
| C65AC1 | GF102022 | GGAGTTGTGCGGTTGAAAGT | 305 | 19 |
| GCACTGCCTAACGGATCATT | ||||
| C65CGC1 | GF102023 | TCTGGGTACAACCCCGTAAC | 221 | 20 |
| AGAGAGTGCGCAAAAGCAAT | ||||
| C65TG1 | GF102021 | ACTGCGAAACGCTTACTGCT | 302 | 9 |
| GTGTGTGGACTGTGGTGGAG | ||||
| C474CT1B | GF110614 | CCCAAACTTGCCACAAAAGT | 290 | 2 |
| CTCACTCTCCGTGAACGACA | ||||
| C48ATC1 | GF102034 | CATTTTTCGGGTGGCTTCTA | 337 | 7 |
| CGAGATCGAAATGATGCTGA | ||||
| C48CGA1B | GF110615 | GCTTGGGAATCTGAATCTGC | 251 | 4 |
| ACCTTGCATTCAACGAGCTT | ||||
| C48GTT1B | GF110616 | GTGGCCACCTGGTTGTAGTT | 309 | 23 |
| ACCACCGGTAGAACATCTCG | ||||
| C175AT1 | GF102036 | GGACCAAGGGTACGATTTGA | 185 | 14 |
| CAGACTGGTTAACGGCTTCC | ||||
| C175TG1 | GF102045 | TCAGATCTCCGAGAGGAGGA | 295 | 4 |
| CTGTCAGGGCCAGATTTCAT | ||||
| C134AC1 | GF102037 | GAAGGTCAGCCACTCAGGC | 194 | 0 |
| ACAGCTGACTCTCGTCGAC | ||||
| C129GT1 | GF102038 | AAGGTGCAAAACCAAACTGG | 377 | 1 |
| TGGAGCACAGCCCTACTCTT | ||||
| C66CA1 | GF102026 | CGACTACTGCCCCAATTTGT | 213 | 2 |
| CACCCTCCCCTACAGACGTA | ||||
| C177CA1B | GF110618 | AGGGCAATGTTTACGACGAC | 293 | 2 |
| CTTGCGCCTTAGTCATCCTC | ||||
| C177TG1 | GF102028 | AGCACAAAAAGGCACGATTT | 197 | 6 |
| TAAACGCAAGTAGGCGGAGT | ||||
| C99TGT1B | GF110619 | GCAGTGGAGGATTCTGAGGA | 358 | 5 |
| CAGAACGTTTGGCGAATTTT | ||||
| C205CA1 | GF102029 | CAATGCGCCTTCTGGATTAT | 227 | 3 |
| CTCGTGATGGCCATTTCTCT | ||||
| C205TG1B | GF110620 | ATTGCTCAAGTGCTGCCTTT | 212 | 7 |
| ATGACGACGAAAAACCGAAC | ||||
| C139TG1B | GF110621 | GGGATCGCTACGTGTTTTGT | 265 | 6 |
| TCTCGGAATGCCAGTCTTTT | ||||
| C446AC2 | GF110622 | CATACGACGTGGAACAAACG | 162 | 17 |
| ACGAGGTTGAGGTTGGTGAC | ||||
| C446TG1 | GF102043 | GGAAAGGGGCACTTGTGTAA | 397 | 22 |
| CGTTTGCTTCTCTTCGAACC | ||||
| CxpGT4 | AY423738 | GTCGTCGCTAACCCTTGTT | 146 | 2 |
| CGCGATAGTCGGTAATCGT | ||||
| CxqTri4 | AY958079 | CTAGCCCGGTATTTACAAGAAC | 121 | 16 |
| AACGCCAGTAGTCTCAGCAG |
aPredicted amplicon size based on nucleotide sequence in VectorBase [50].