Table 2.
Fusions recurring in the same cellular location
Total | Single | T–T | T–F | F-T | F-F | >2 fusions | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Condition | n | n | % | n | % | t (s) | n | % | t (s) | n | % | t (s) | n | % | t (s) | n | % |
Spontaneous | 349 | 271 | 77.7 | 54 | 15.5 | 1.28 | 5 | 1.4 | 11.55 | 7 | 2.0 | 11.57 | 5 | 1.4 | 14.57 | 7 | 2.0 |
Bradykinin | 1572 | 1315 | 83.7 | 106 | 6.7 | 1.95 | 33 | 2.1 | 7.70 | 34 | 2.2 | 12.10 | 55 | 3.5 | 16.71 | 29 | 1.8 |
ATP | 524 | 378 | 72.1 | 68 | 13.0 | 0.88 | 17 | 3.2 | 0.16 | 20 | 3.8 | 5.05 | 23 | 4.4 | 14.47 | 18 | 3.4 |
4-Br-A23187 | 897 | 775 | 86.4 | 78 | 8.7 | 1.86 | 9 | 1.0 | 5.13 | 12 | 1.3 | 7.97 | 13 | 1.4 | 17.58 | 10 | 1.1 |
α-Latrotoxin | 426 | 346 | 81.2 | 58 | 13.6 | 1.66 | 3 | 0.7 | 13.51 | 9 | 2.1 | 1.04 | 3 | 0.7 | 19.53 | 7 | 1.6 |
Mechanical | 321 | 244 | 76.0 | 59 | 18.4 | 0.20 | 4 | 1.2 | 0.13 | 3 | 0.9 | 0.23 | 2 | 0.6 | 18.85 | 9 | 2.8 |
Sucrose | 558 | 383 | 68.6 | 129 | 23.1 | 0.86 | 2 | 0.4 | 20.50 | 16 | 2.9 | 7.22 | 7 | 1.3 | 18.71 | 21 | 3.8 |
Average | 78.0 | 14.1 | 1.24 | 1.4 | 8.38 | 2.2 | 6.45 | 1.9 | 17.21 | 2.4 |
Locations in astrocytes where two or more fusions occurred in the same spot were counted and the type of fusion (T, transient; F, full) and order they occurred were recorded. The number of fusions (n) along with the per cent (%) of the total fusions are shown in each column. As most repeat fusions consisted of 2 fusions in the same spot we show only the first 2 fusions in the sequence. The number of repeat fusions greater than 2 events is shown to the far right. We also measured the time interval (t), in seconds, between recurring fusions. The bottom row reports the average proportion of total fusions that occurred in each particular order, and the average time between two fusions.