Fig. 5.

Developmental and tissue-specific expression of the mAsr family members. Relative mRNA abundance of mAsr1 to mAsr4 was measured by quantitative RT-PCR. For each member of the mAsr family, mean relative abundance values were calculated and compared to each other on a pair-wise basis using Student’s t tests. Standard errors are represented. Comparisons that were significant are indicated by one, two or three asterisks depending on whether the P value was <0.05, <0.01 or <0.001, respectively. a Comparison of mRNA abundance in greenhouse leaves and meristem cultures of the ‘Williams’ (AAA) cultivar. The two reference genes used for normalization were EF1α and 25S r-DNA. Values obtained for leaves (gray bars) are expressed relative to values obtained for meristem cultures (black bars). b Comparison of mRNA abundance in the first (light gray bars) and second leaves (dark gray bars) as well as root tissue (white bars) from greenhouse plants of the ‘Cachaco’ (ABB) cultivar. The two reference genes used for normalization were EF1α and L2