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. 2011 Sep 7;12:38. doi: 10.1186/1471-2121-12-38

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Copyright ©2011 Prexl et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Co-immunofluorescence staining with anti-JAG-IC- and anti-ZIC7A12-antibodies. Single confocal sections of a hydra from a region of the body column: (A, E, I, M, Q) DNA staining with TO-PRO3, (B, F, J, N, R) anti-JAG-IC staining, (C, G, K, O, S) anti-ZIC7A12 staining, (D, H, L, P, T) merged images of all channels in false colours: DNA (blue), anti-JAG-IC staining (green), anti-ZIC7A12 staining (red); (A-D) overview of the staining pattern in the body column of an adult hydra, (E-H) localization of HyJagged in single (s) and a pair of interstitial cells (p), (I-L) staining of a nest of four nematoblasts (n), (M-P) nest of nematoblasts with vacuoles (v), (Q-T) staining of mature nematocytes (nc); scale bars: (A-D) 10 μm, (E-T) 5 μm.