Table 1.
Purification of PGC2 from culture of FOC4 grown on SM supplemented with 1% citrus pectin as the sole carbon source
| Step | Total protein (mg) |
Total activity (Unit)a |
Yield (%) |
Specific activity (Units mg protein-1min-1) |
|---|---|---|---|---|
| Crude | 36.60 | 131.25 | 100 | 3.59 |
| Ultrafiltration | 8.21 | 70.26 | 53.53 | 8.56 |
| Sephacryl S-100 16/60 | 1.85 | 20.35 | 15.51 | 11 |
| Sepharose FF CM Hitrap | 0.26 | 5.54 | 4.22 | 21.30 |
a One unit of PG activity was defined as the amount of enzyme that liberated one μmole of GA in one minute at 45°C.