Table 2.

A panel of meiotic genes and their expression patterns in ameiotic1 (am1) anthers.

Maize Gene ID	Names	Homolog	Stage	qRT Verified	Differentially Expressed
GRMZM2G075563	Ms5	plant only	various		down in am1-489 at 1.5 mm
GRMZM2G059037	Afd1	Rec8/Rad21	prophase I	√	down in am1-489 no change in am1-praI at both stages
GRMZM5G883855	Am1	plant only	pre-meiotic	√	down in am1-489; up in am1-praI at both stages
GRMZM2G109618	Dmc1	Dmc1	prophase I	√	down in both mutant alleles at 1.5 mm
GRMZM2G408897	Mmd1	plant only	early-late		down in both mutant alleles at 1.5 mm
GRMZM2G060394	Skp1A	Skp1A	prophase I	√	significantly down in both mutant alleles at 1.5 mm
GRMZM2G032562	Skp1B	Skp1B		√	significantly down in both mutant alleles at 1.5 mm
GRMZM2G143590	Zyp1	Zip1	prophase I	√	down in both mutant alleles at both stages
GRMZM2G083138	Rad54-like	Rad54-like	prophase I	√	down in both mutant alleles, particularly at 1.5 mm
GRMZM2G100103	Phs1	Rec114	prophase I		low or no expression in all
GRMZM2G129913	Spo11-1	Spo11	prophase I	nc	marginal changes
GRMZM2G454838/GRMZM2G328795	Zip4/Spo22-like	Zip4/Spo22-like	prophase I		down in both mutant
GRMZM5G883855	Aml1	Mei2	prophase I		no changes
GRMZM2G102242	Mnd1	Mnd1	prophase I		no changes
GRMZM2G308884	Prd1	Mei1	prophase I		no changes
GRMZM2G121543	Rad51A	Rad51	prophase I	√	no changes
GRMZM2G084762	Rad51A2	Rad51A2	prophase I	√	no changes
GRMZM2G115013	RPA70	RPA1a	various	√	down in am1-489 at 1.0 and 1.5 mm
GRMZM2G315902	Mlh3	Mlh3	prophase I		no changes or not significant
GRMZM2G055899	Pair1a	plant only	pre-meiotic		not significant
GRMZM2G056075	Msh5	Msh5	various		not significant except up in am1-489 at 1.5 mm
GRMZM2G457370	Ago1	Ago1	pre-meiotic		up in both mutant alleles at both stages

√: qRT analyses found the same direction of regulation and approximately the same magnitude of change as calculated from the microarray analysis; nc: qRT and array results were non-congruent.