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Reactivity of serum from a BNP-dam with leukocytes from different animals. Immunoprecipitation was performed with serum of a BNP-dam on cell surface biotinylated leukocytes from a susceptible calf (lane 1), a different BNP-dam (lane 2) and a non-BNP-dam, which was vaccinated with PregSure® BVD, but was tested negative for MHC I alloantibodies (lane 3). The 44 kDa and 12 kDa protein (arrows) were only recognized in the susceptible calf and the non-BNP-dam.
