Table 3.
ABP minimal cut sets (MCSs) with corresponding ABP gene(s) and reactions.
| MCS | N-3-D-RXN | LEUCPEL-RXN | D-4-R-RXN | RXN-525 | APIGNAR-RXN | RXN-600 | RXN-602 | RXN-7652 | PELUDP-RXN | RXN1F-775 | RXN-7775 | N-C-S-RXN | Total rxns | Unaffected | Genes/enz in MCS |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | CHI |
| 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | CHS |
| 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 57 | F3H, DFR(cy) |
| 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 9 | F3H, F3'H(i) |
| 5 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 126 | ANS(pg), DFR(cy) |
| 6 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 123 | DFR(pg), DFR(cy) |
| 7 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 60 | F3H, ANS(cy) |
| 8 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 132 | ANS(pg), ANS(cy) |
| 9 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 129 | DFR(pg), ANS(cy) |
| 10 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 129 | DFR(cy), 3-UGT(pg) |
| 11 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 135 | ANS(cy), 3-UGT(pg) |
| 12 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 63 | F3H, 3-UGT(cy) |
| 13 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 138 | ANS(pg), 3-UGT(cy) |
| 14 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 135 | DFR(pg), 3-UGT(cy) |
| 15 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 141 | 3-UGT(pg), 3-UGT(cy) |
| 16 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 18 | F3H, F3H(cy) |
| 17 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 39 | ANS, F3'H, F3'H |
| 18 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 36 | DFR, F3'H, F3'H |
| 19 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 42 | F3'H, F3'H, 3-UGT(pg) |
| 20 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 48 | ANS, F3'H, F3H(cy) |
| 21 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 45 | DFR, F3'H, F3H(cy) |
| 22 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 51 | F3'H(ii), 3-UGT(pg), F3H(cy) |
| fc | 0.50 | 0.43 | 0.43 | 0.33 | 1 | 0.50 | 0.50 | 0.38 | 0.43 | 0.50 | 0.38 | 1 | |||
| Enzymes | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |||
Table 3 shows the set of MCSs or ABP gene candidates for genetic changes needed to eliminate anthocyanin production. Some reactions in the top row have been abbreviated and the numbers in the bottom row represent enzymes corresponding to the reactions in the top row; the details are as follows:
Reactions (top row):
N-3-D-RXN: NARINGENIN-3-DIOXYGENASE-RXN
D-4-R-RXN: DIHYDROKAEMPFEROL-4-REDUCTASE-RXN
N-C-S-RXN: NARINGENIN-CHALCONE-SYNTHASE-RXN
Unaffected: Non-affected flav EMs
Enzymes (bottom row):
1: F3H; 2: ANS/LDOX (pg); 3: DFR(pg); 4: F3'H(ii); 5: CHI; 6: DFR(cy); 7: ANS/LDOX (cy); 8: F3'H(i); 9: 3-UGT/UDPG (pg); 10: 3-UGT/UDPG (cy); 11: F3H(cy); 12: CHS
The reactions directly forming the anthocyanin compounds (PELUDP-RXN and RXN1F-775) are identified as the objective functions for which the MCSs are determined. In the last column, parallel enzymes are listed by a single representative: ANS & LDOX corresponding to LEUCPEL-RXN as ANS(pg), ANS & LDOX corresponding to RXN-602 as ANS(cy); 3-UGT & UDPG corresponding to PELUDP-RXN as 3-UGT(pg) and 3UGT & UDPG corresponding to RXN1F-775 as 3-UGT(cy). fc - fragility coefficient. (Refer to Figure 3 legend or List of abbreviations used, for explanation of the abbreviated enzymes.)