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. 2011 Jun 7;43(15):930–941. doi: 10.1152/physiolgenomics.00069.2011

Table 2.

Primer sequences used for cloning the Prl5a1 5′-flanking region

Primer Amplicon Primer Sequence
Prl5a1-953 forward (−5048) −5048 to +63 AGTGGAGACTCCCAGCTCAA
Prl5a1-6063 reverse (+63) −5048 to +63 AGGGATGTGGTTGAATCTGC
2349XhoI-forward (−3650) −3650 to +63 GCTTGGTGGCACATCTCGAGACTT
5050Xho-forward (−951) −951 to +63 GGGCTCTAAAGGCTCGAGGA
6063NcoI-reverse (+63) −3650 to +63 and −951 to +63 CTTACAGGGCCATGGTTGAATCTG