Fig. 1.

Frequency of the novel low-density lipoprotein receptor (LDLR) cDNA in rhesus macaque liver tissues. A: partial genomic sequence of exon 4, the boundary intron sequence, and exon 5. Cartoon diagram depicting site of alternative splice event. B: AlignW analysis comparing reference cDNA sequences of human (GenBank accession no. BT007361), mouse (GenBank accession no. Z19521), and rhesus (rh) LDLR (GenBank accession no. AY466854) to the novel rhesus LDLR described in this paper. C: exon 4 and 5 was amplified from cDNA of normal wild-type rhesus macaques. The PCR products were size-fractionated, gel-purified, and digested with MscI. PCR products from the RhLDLR-21 gene are not digested by MscI. The 21 bp insertion in exon 5 creates a new MscI site. MscI digestion of the PCR products from the novel rhLDLR gene generates fragments of 381 and 123 bp. Numbers above the lanes identify the individual ID of the rhesus macaque. cDNA from a total of 14 rhesus macaques and peripheral blood mononuclear cells (PBMCs) from 5 familial hypercholesterolemia (FH) rhesus macaques were analyzed using this approach.