Fig. 2.

Prevalence of rhLDLR and rhLDLR-21 homologs in human liver tissues. Exons 4 and 5 were amplified from cDNA isolated from humans. The PCR products were size-fractionated, gel-purified, and digested with MscI. PCR products lacking the 21 bp sequence coinciding with exon 5 are not digested by MscI. The 21 bp insertion in exon 5 creates a new MscI site. MscI digestion of PCR products containing the 21 bp insertion generates fragments of 381 and 123 bp. Numbers above the lanes identify the individual ID of the human (H), wild-type rhesus (R), and FH rhesus macaques (F). *Nondigested fragment from this sample was subsequently cloned and sequenced. cDNA from a total of 12 human liver samples were analyzed using this approach.