Fig. 7.

Altered promoter activity with SNPs in or near CArG boxes. Luciferase activity measured 24 h after transfection of the indicated reporters encompassing either WT or SNP sequence constructs into COS7 cells in the absence or presence of SRF-VP16 (see materials and methods). Data were normalized to a control Renilla reporter that was cotransfected in all samples. *Statistical significance between WT and SNP in the presence of SRF-VP16. Error bars show SD, n = 4. Similar results were found in independent transfections using another cell type. Boldfaced gene names indicate CArGs that conform to the consensus sequence, while lightfaced gene names are CArG-like sequences that deviate from the consensus sequence by 1 bp. 909 represents the empty vector control.