Table 1.
Husbandry Parameters at Each Lab
| Wild type used | Fry feeding regime | Spawning adult feeding regime | Age of visual sexual dimorphism | Age when consistent spawning begins | Mortality rate at 0–1 month | Average adult mortality over 3 months | Fry stocking density | Adult stocking density | System manufacturer and tank size | Breeding cages used for study | H2O exchange rate as tank changes per hour | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Lab 1 | EK | Hatchfry Encapsulon, AP100 and decapsulated artemia | Zeigler crumble twice/day decapsulated artemia once/day | 3 months | 4 months | 30% | ∼1% | Up to 30 fry/L | 7–10 fish/L | Marine Biotech Z-mod 2L tanks | Techniplast 0.75 L | 3–4 |
| Lab 2 | AB | Hatchfry Encapsulon, AP100, and decapsulated artemia | Zeigler crumble twice/day decapsulated artemia once/day | 3 months | 4 months | 30% | ∼1% | Up to 30 fry/L | 7–10 fish/L | Marine Biotech Z-mod 2L tanks | Techniplast 0.75 L | 3–4 |
| Lab 3 | TuAB | Hatchfry Encapsulon and decapsulated artemia | decapsulated artemia twice/day, Flake (omega one or spirulina, krill, plankton flake) | 2.5 months | 3.5 months | 10–20% | <5% | Up to 15 fry/L | 2–3 fish/L (9 L tanks) | AHAB 1, 2.75, and 9 L tanks | AHAB 1 L | 6 |
| Lab 4 | AB X TL F1 hybrid | Paramecium and/or decapsulated artemia + powered dried food gape size appropriate 3 times/day | Various pelleted food + freeze dried plankton and decapusalted artemia 3 times/day | 3–4 months | 4 months | 15% | <1% | Up to 16 fry/L | 5 fish/L | AHAB 10 and 3L tanks | AHAB 1 L | 6 |
| Lab 5 | AB | Artemia 4 times/day, Liquified mix spirulina and encapsulon 2–3 times/day | Artemia enriched with Selco or spirulina twice/day, Lansey Pellet with spirulina twice/day | ∼2 months | 5 months | 25% | 10% | Up to 20 fry/L | 3–4 fish/L (11 L tanks) | Aquarienbau Schwarz 1.8 and 11 L tanks | 2 L mouse cages | 4 |
| Lab 6 | AB | Sera Micron (ad libitum) Artemia 4 times/day or Proton pellets | pellets 3 times/day decapsulated artemia once/day | 1.5–2 months | 3.5–4 months | 10–20% | <10% | 20–25 fry/L | 5 fish/L | Aquaneering 1, 2, and 9 L tanks | AHAB 1 L | 8 |
| Lab 7 | AB | Nannochloropsis, Hatchfry Encapsulon, paramecia, and decapsulated artemia 3 times/day | Cyclop-eeze 3 times/week, decapsulated artemia once/day, Zeigler crumble once/day | 1.5 months | 2.5–3 months | <10% | Not measured | 30 fry/L reduced to 7 fry/L at day 20 | 8 fish/L | AHAB 1.5, 2.75, and 10 L tanks | AHAB 2 L (filled to 1 L) | 5–6 |
| Lab 8 | AB | Paramecia 3 times/day, INVE proton pellet daily, artemia 2 times/day | dry food once daily (INVE food 6/8 (600 to 1000 μm) and PerlaAq) once daily decapsulated artemia | 3 months | 3.5–4 months | <10% | <1% | 60 fry/500 mL | 4 fish/L | AHAB 1.5, 2.75, and 10 L tanks | AHAB 2 L | 5–6 |
Underlined and italicized text represents conditions that were specifically used in the present study. All other entries apply to general husbandry practices at each institute.