Figure 7.

Phosphomimic Substitution at Thr-242 in the AD of BIK1 Confers Enhanced ET Responses and PTI to B. cinerea.

(A) In vivo kinase activity of the BIK1^T242D and BIK1^Y245D substitution mutants in response to ACC (Ac) and flg22 (Fl). Kinase activity of the phosphomimic mutants visualized by audioradiograms of radiolabeled proteins following incubation with [γ-³²P]ATP.

(B) Constitutive activation of BIK1 phosporylation by the BIK1^T242D phosphomimic mutation. BIK1 phosphorylation was visualized as a mobility shift detected by HA-immunoblot. Coomassie blue was used as a loading control.

(C) Lesion diameter in water (−) and flg22 (+) pretreated plants following drop inoculation with B. cinerea (2.5 × 10⁵ spores/mL). Wt, wild type.

(D) and (E) Triple response (D) and average hypocotyl length (E) of seedlings on different concentrations of ACC (μM) (+) or unsupplemented MS media (−).

In (A), protein staining with Coomassie blue was used as a loading control. Data in (C) and (E) represent mean ± se from a minimum of 30 disease lesions or 60 seedlings, respectively. Experiments were repeated at least three times with similar results. Statistical analysis was performed as described in the legend of Figure 2.

[See online article for color version of this figure.]