Figure 4.

RopGEF7 Interacts with and Activates At RAC/ROPs.

(A) to (C) Arabidopsis protoplasts were cotransfected with N-terminal YFP and C-terminal YFP (A), nYFP-At RAC1 and cYFP-RopGEF7 (B), and nYFP-At RAC1 and cYFP-GEF7ΔC (C). Images are acquired under the YFP and differential interference contrast (DIC) channel, respectively, and then merged together.

(D) to (F) Localization of YFP-labeled At RAC1 (D), RopGEF7 (E), and GEF7ΔC (F).

(G) GST-PBD pull-down assays for active At RACs in wild-type seedlings and RPS5A_pro:RopGEF7ΔC-overexpressing line L34 (top panel). Bottom panel shows total At RAC/ROPs (active and inactive) from the corresponding samples. Nt Rac1 antibody was used against At RAC/ROP proteins.

Bars = 10 μm in (A) to (F).