Figure 5.

Upregulation of RAC/ROP Signaling Induces the Ectopic Production of Root-Like Structures in RopGEF7ΔC-Overexpressing Seedlings.

(A) Relative expression levels of RopGEF7ΔC, as determined by qRT-PCR, in three transgenic lines compared with the wild type. Data presented are mean values of four biological repeats with sd.

(B) Seven-day-old wild-type seedling.

(C) and (D) Ectopic production of root-like structures from cotyledon tips in 10-d-old (C) and 7-d-old RPS5A_pro:RopGEF7ΔC seedlings (D).

(E) and (F) Ectopic production of root-like structures near the shoot apex in 7-d-old RPS5A_pro:RopGEF7ΔC seedlings.

(G) and (H) Expression of PLT1_pro:PLT1:YFP in wild-type (G) and RopGEF7ΔC (H) embryos.

(I) and (J) Expression of PLT2_pro:PLT2:YFP in wild-type (I) and RopGEF7ΔC embryos (J).

(K) Expression of PLT1_pro:PLT1:YFP in root-like structures emerging from cotyledon tips in 7-d-old RopGEF7ΔC seedlings.

(L) Expression of PLT2_pro:PLT2:YFP in root-like structures emerging from cotyledons and the main root of 7-d-old RopGEF7ΔC seedlings. Arrowheads point to root-like structures. Arrows indicate the expression of the marker genes in the root-like structures.

Bars = 0.5 mm in (B) to (F), 20 μm in (G) to (J), and 200 μm in (K) and (L).