Expression of DR5:GUS and Effects of Exogenous Auxins.
(A) to (F) Expression pattern of the DR5:GUS reporter gene in young seedlings. Expression of DR5:GUS in the seedlings of the wild type (A) and fugu5-1 mutant (B) at 6 DAS. Expression of DR5:GUS in wild-type root and lateral root primordium at 6 DAS, respectively ([C] and [D]). Expression of DR5:GUS in fugu5-1 mutant root and lateral root primordium at 6 DAS, respectively ([E] and [F]). DR5:GUS signals around root apical meristem (black arrowheads), in the vascular system (open arrowheads), and in the emerging lateral root primordia (asterisk). Bars = 2 mm in (A), 1 mm in (B), and 100 μm in (C) to (F).
(G) Effect of exogenous auxin on the growth of fugu5 mutant. Seedlings of the wild type (WT) and fugu5-1 grown on MS alone, MS + 2% Suc, MS + indole-3-acetic acid (5 μM), and MS + 1-naphthaleneacetic acid (5 μM) 8 at DAS. Bar = 2 mm.
(H) Effect of exogenous auxin on cell number in the cotyledons. Average cell numbers of samples described in (G) were determined. Data are means and sd (n = 8). NS, no significant difference between the two genotypes (the wild type and fugu5-1). Asterisk indicates significant difference at P < 0.01.