Figure 1.

msl2 msl3 Double Mutant Chloroplasts Contain Multiple Z-Rings.

(A) Representation of MSL2 and MSL3 coding regions. Exons are indicated by black bars and introns by lines. The T-DNA insertion sites in msl2-3, msl2-1, and msl3-1 alleles are indicated.

(B) Immunofluorescence micrographs of FtsZ1 ring placement in chloroplasts isolated from rosette leaves. Arrowheads indicate Z-rings, shown in green. Chlorophyll autofluorescence is pseudocolored red. Bars = 10 μm.

(C) Quantitative analysis of isolated chloroplasts containing single or multiple Z-rings. The average of three independent experiments ± the se of the means is presented. n ≥ 100 chloroplasts, per replicate.