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. 2011 Aug 2;23(8):2939–2949. doi: 10.1105/tpc.111.088112

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© 2011 American Society of Plant Biologists. All rights reserved.

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Figure 4. — msl2 msl3 arc11 Triple Mutants Are Phenotypically Indistinguishable from Single arc11 Mutants.

(A) Immunofluorescence micrographs of Z-ring placement in chloroplasts isolated from rosette leaves of the indicated genotypes. FITC and chlorophyll fluorescence are pseudocolored green and red, respectively. Bars = 10 μm.

(B) Quantitative analysis of Z-rings in isolated chloroplasts subjected to IFM. The average values from two independent experiments ± se of the means are presented. n ≥ 100 chloroplasts per replicate.

(C) The distribution of Z-ring number in chloroplasts of the indicated genotypes. Z-rings of isolated chloroplasts were visually counted and percentage of chloroplasts containing one to nine Z-rings was calculated. n ≥ 50 chloroplasts per genotype.