msl2 msl3 arc11 Triple Mutants Are Phenotypically Indistinguishable from Single arc11 Mutants.
(A) Immunofluorescence micrographs of Z-ring placement in chloroplasts isolated from rosette leaves of the indicated genotypes. FITC and chlorophyll fluorescence are pseudocolored green and red, respectively. Bars = 10 μm.
(B) Quantitative analysis of Z-rings in isolated chloroplasts subjected to IFM. The average values from two independent experiments ± se of the means are presented. n ≥ 100 chloroplasts per replicate.
(C) The distribution of Z-ring number in chloroplasts of the indicated genotypes. Z-rings of isolated chloroplasts were visually counted and percentage of chloroplasts containing one to nine Z-rings was calculated. n ≥ 50 chloroplasts per genotype.