Figure 7.

Photosynthetic Electron Transfer Is Involved in the Regulation of LHCSR3 Expression.

(A) Expression of LHCSR3 requires active photosynthetic electron transfer. Wild-type cells (WT; cw15-arg7) in the presence and absence of DCMU (20 μM) or DBMIB (20 μM) as well as nac2 (PSII-deficient mutant; Kuchka et al., 1989) and Δpsab (PSI-deficient mutant; Redding et al., 1998) mutant cells were exposed in HL for 2 h in 80% HSM. Subsequently, whole-cell extracts (2.5 μg of chlorophyll) were fractionated on a 13% SDS-PAGE gel, and LHCSR3 abundance was analyzed by immunoblots. CF1 signal served as a loading control.

(B) Impact of nigericin (0.25 to 1.0 μM) in LHCSR3 expression in wild-type cells (CC-124) during a 2-h HL shift experiment in 80% HSM. Protein analyses were performed as described in (A).