Figure 2.

Interference with Post-Golgi Rab GTPase Activity Highlights a Single Route to the Vacuole for ssVSS and ctVSS Cargo.

Tobacco protoplasts were cotransfected with a constant amount of plasmid (15 μg) encoding either ssVSS cargo amy-spo (A) and Aleu-amy (B) or the ctVSS cargo amy-chi (C) and amy-BN2SA (D) alone (first lane) or together with increasing concentrations of either Rab11(NI), Rha1(NI), Ara6(NI), and Rab7(NI) harboring plasmids as indicated below each lane. α-Amylase assays and routine verification of ST-YFP by immunoblotting was performed as described in Figure 1. Error bars represent standard errors from five independent transfections for each condition.