Figure 5.

RFP-Chi Transport Shows the Same Sensitivity toward Rab Mutants as Aleu-RFP Does.

The trafficking of the ctVSS fluorescent chimera RFP-chi was tested against the same Rab mutants using experimental conditions as in Figure 4 and subjected to the same statistical testing. In the presence of the mock effector PAT, RFP-chi is partially detected in the central vacuole (A) and in transit through the ER (B) as well as small extra-Golgi punctae ([B]; indicated by arrows). Coexpression with Rab11(NI) shows RFP-chi mostly in the ER without punctate post-Golgi structures (C). Rha1(NI), Ara6(NI), and particularly Rab7(NI) caused a strong accumulation of the chimera in post-Golgi structures ([D] to [F]). Bars = 10 μm.