Figure 7.
αTIP-YFP but Not CBL6-RFP Trafficking Is Rha1, Ara6, and Rab7 Dependent.
Tobacco leaves were coinfiltrated with Agrobacterium strains encoding αTIP-YFP together with the various dual expression vectors for CBL6-RFP, and the various Rab mutants and annotations are as before. Notice that none of the effectors alters the tonoplast localization of CBL6-RFP, while αTIP-YFP is blocked in punctate structures en route to the tonoplast upon coexpression with Rha1(NI) (C), Ara6(NI) (D), or Rab7(NI) (E). Bars = 10 μm.