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. 2011 Aug 19;23(8):3007–3025. doi: 10.1105/tpc.111.085480

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Figure 9. — The Mobile Clusters Contain Markers for the TGN, the PVC, and the Vacuole but Not CBL6-RFP.

Tobacco leaves were infiltrated with an Agrobacterium strain encoding GFP-Vam3 together with new double vectors containing Rab7(NI) as effector and either the TGN marker YFP-SYP61 (A), the PVC marker RFP-VSR2 (B), the soluble vacuolar marker Aleu-RFP (C), or the tonoplast-localized CBL6-RFP (D). YFP-SYP61 (A) and RFP-VSR2 (B) redistribute into the same mobile clusters in the presence of GFP-Vam3 and Rab7(NI). The soluble vacuolar marker Aleu-RFP is also observed in these putative TGN-PVC supercompartments (C). GFP-Vam3 is trapped in the clusters in the presence of Rab7(NI) but these exclude CBL6-RFP (white arrowheads) as the CBL6 fusion still reaches the vacuolar membrane (D). Bars = 10 μm.