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. 2011 Aug 2;23(8):3026–3040. doi: 10.1105/tpc.111.087254

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© 2011 American Society of Plant Biologists. All rights reserved.

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Figure 9. — AMSH3 Competes with SKD1 for VPS2.1 Binding.

(A) SKD1 in vitro binding assay. GST, GST-VPS2.1, and GST-VPS24.1 loaded on GST Sepharose 4B beads were incubated with His-SKD1. After extensive washing, the bead-bound materials were analyzed by immunoblotting using anti-His and anti-GST antibodies.

(B) SKD1 competition assay. Binding assay of GST-2.1 or GST-24.1 with 6 pmol of AMSH3 was performed as in Figure 1C in the presence of 6, 72, and 216 pmol of His-SKD1. Note that AMSH3 binding becomes weaker in the presence of an excess amount of SKD1.

(C) Predicted model for the AMSH3–ESCRT-III interaction. AMSH3 associates with ESCRT-III through its binding to VPS2.1 and VPS24.1. AMSH3 competes with SKD1 for the binding to VPS2.1 MIM1, but probably not to VPS20. The timely disassociation of AMSH3 may be necessary for SKD1 to be able to bind efficiently and disassemble ESCRT-III.