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. 2011 Aug 23;23(8):3055–3069. doi: 10.1105/tpc.111.086827

Figure 5.

Figure 5.

The Product of the A. aestivalis cbfd2 cDNA, Earlier Shown to Add a Hydroxyl to the Number 4 Carbon of Carotenoid β-Rings (Cunningham and Gantt, 2005), Adds a Hydroxyl to the Number 3 Carbon of 4-Keto-β-Rings.

Shown are HPLC elution profiles for extracts of E. coli cultures wherein cells contained the following: (A) pAC-CANTHipi; (B) pAC-CANTHipi and pCBFD2; and (C) pAC-CANTHipi and pHBFD1Bad. Absorption spectra for the peaks in (A) are displayed in Supplemental Figure 6 online. Absorption spectra for the peaks in (B) and (C) are shown in Supplemental Figure 7 online. Arabinose (0.05% [w/v]) was included in the growth medium of the E. coli culture wherein cells contained pHBFD1Bad. Numbers in parentheses are HPLC retention times in minutes. Those peak identifications followed by question marks in (B) and (C) are speculative: They are consistent with absorption spectra, HPLC retention times, and the known catalytic capabilities of the carotenoid pathway enzymes that are present in the E. coli cells, but the requisite standards were not available for comparison.