Table 1.
Plasmids Used in This Work
| Plasmid | Description | Source or Reference |
| pBluescript SK− | cDNA library vector; pUC origin; Ampr | Stratagene |
| pSTBlue-1 | Blunt cloning vector; pUC origin; Ampr; Kanr | Novagen |
| pBAD/His A | Expression vector; arabinose inducible; pBR322 origin; Ampr | Invitrogen |
| pBAD/His B | Expression vector; arabinose inducible; pBR322 origin; Ampr | Invitrogen |
| pCBFD1 | Previously called pAdKeto1, pUC origin; Ampr | Cunningham and Gantt (2005) |
| pCBFD2 | Previously called pAdKeto2, pUC origin; Ampr | Cunningham and Gantt (2005) |
| pCBFD1/CBFD2 | Previously called pAdKeto1/2, contains A. aestivalis cDNAs cbfd1 (AdKeto1) and cbfd2 (AdKeto2), pUC origin; Ampr | Cunningham and Gantt (2005) |
| pCBFD2Bad | Previously referred to as pAdKeto2Bad; contains the A. aestivalis cbfd2 cDNA with expression under control of the araBAD promoter; pBR322 origin; Ampr | Cunningham and Gantt (2005) |
| pAC-BETA | Produces β-carotene in E. coli; p15A origin; Capr | Cunningham et al. (1996) |
| pAC-BETAipi | pAC-BETA with an Erwinia herbicola ipi gene added to improve yield; p15A origin; Capr | Cunningham and Gantt (2005) |
| pAC-BETA-CBFD1/2 | pAC-BETA with the A. aestivalis cbfd1 and cbfd2 cDNAs inserted; ~13.6 kb; p15A origin; Capr | This work |
| pAC-CANTHipi | Produces canthaxanthin in E. coli; some echinenone and β-carotene also accumulate; p15A origin; Capr | Cunningham and Gantt (2007) |
| pHPK | H. pluvialis cDNA library plasmid; the cDNA encodes a carotenoid β-ring 4-ketolase (BKT); pUC origin; Ampr | Lotan and Hirschberg (1995) |
| pHBFD1 | A. aestivalis cDNA library plasmid, pUC origin; Ampr | This work |
| pHBFD2 | A. aestivalis cDNA library plasmid, pUC origin; Ampr | This work |
| pHBFD1Bad | hbfd1 cDNA cloned, in frame, in pBAD/His B; expression under control of the araBAD promoter; ~5.3 kb; pBR322 origin; Ampr | This work |
| pCBFD1/HBFD1Bad | cbfd1 cDNA inserted in pHBFD1Bad; ~6.9 kb; pBR322 origin; Ampr | This work |
| pCBFD2Bad/HBFD1 | A. aestivalis cbfd2 cDNA, fused to the araBAD promoter, and araC gene were together inserted into pHBFD1; ~6.7 kb; pUC origin; Ampr | This work |
| pNaHBFD-likeBad | N. advena cDNA encoding a polypeptide similar to the A. aestivalis HBFD polypeptides cloned in frame in pBAD/His A; expression under control of the araBAD promoter; ~5.6 kb; pBR322 origin; Ampr | This work |
| pCBFD1/NaHBFD-likeBad | cbfd1 cDNA inserted in pNaHBFD-likeBad; ~7.2 kb; pBR322 origin; Ampr | This work |
Ampr, ampicillin resistance; Capr, chloramphenicol resistance; Kanr, kanamycin resistance.