Analysis of Sapogenin Content in Leaves and Roots of lha-1 Mutant Line and Control Line.
(A) GC-MS chromatograms of sapogenins in roots: lha-1 mutant (1) and control line (E113) (2).
(B) and (C) Sapogenin content obtained by GC-FID analysis in leaves and roots, respectively; values for lha-1 (gray bars) and the control line (white bars) are means ± se of three biological replicates (three to 10 plants/replicate). Identification of soyasapogenol B was achieved considering all the artifactual compounds detected (soyasapogenols C, D, and F). *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001 with F test).
bayo, bayogenin; DM, dry matter; hed, hederagenin; IS, internal standard; med, medicagenic acid; olea, oleanolic acid; soya, soyasapogenol; zan, zanhic acid.