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. 2011 Aug 5;23(8):3070–3081. doi: 10.1105/tpc.111.087312

Figure 3.

Figure 3.

Identification of the T-DNA–Tagged Locus and Expression of the CYP716A12-Tagged Gene in the lha-1 Mutant.

(A) Location of the T-DNA insertion in CYP716A12 gene (not drawn to scale). The relative location and orientation of the T-DNA are shown. The T-DNA right border (RB) with four copies of the 35S enhancer was inserted at the end of the first exon producing the loss of small parts of the exon (in white) and the intron (hatched). LB, left border; UTR, untranslated region.

(B) RT-PCR analysis of transcript level of CYP716A12 gene in leaves and roots of the control line (E113) and the lha-1 mutant. C-, negative control; Msc27, reference gene.