Figure 5.

Sapogenin Content in Mutant and Control Plants.

Data were obtained by GC-FID analysis of lha-2 (CYP2768) and lha-3 (CYP3201) mutant lines (M3 generation) from a TILLING collection and the respective control plants.

(A) lha-2: Mutant (gray bars, nine plants) and control Jemalong 2HA10-9-3 (black bars, three plants).

(B) lha-3: Homozygous mutant (gray bars, three plants), heterozygous mutant (white bars, three plants), and wild type (black bars, three plants).

Values are means ± se. (*P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001 with F test and linear contrasts).

bayo, bayogenin; DM, dry matter; med, medicagenic acid; soya, soyasapogenol; spg, sapogenins; wt, wild type; zan, zanhic acid.